The savannah area around the pan is home to herds of elephants and migratory birds as well as flamingos, zebra, blue wildebeest and springbok, the endangered black rhinoceros, lions, leopards and antelopes. The greater park is home to several hundred species of mammals, reptiles and birds.
Close to Twyfelfontein lies two interesting features, Burnt Mountain and the 'Organ Pipes', a geological feature caused by weathering around dolorite pillars. Burnt Mountain get its name from the areas of black limestone that surround the base.On the southern border of the park lies the Ondundozonananandana hills (Leopard Hills). This striking name translates as ‘place where young boy herding cattle went to never return’ - an indication of the number of cat predators including leopards in the area. Most of the park is savanna woodlands except for areas close to the pan, with sandveld in the north eastern corner containing Acacia and Terminalia trees in abundance.
Visitors on safari can expect to see an abundance of African elephant, giraffe, lions and leopards as well as numerous other species in abundance. More rarely seen are the black rhinoceros and white rhinoceros.